Sugar nucleotides from the fungus Aureobasidium.

The halophilic fungus Hortaea werneckii and the halotolerant fungus Aureobasidium pullulans maintain low intracellular cation concentrations in hypersaline environments.

Hortaea werneckii and Aureobasidium pullulans, black yeast-like fungi isolated from hypersaline waters of salterns as their natural ecological niche, have been previously defined as halophilic and halotolerant microorganisms, respectively. In the present study we assessed their growth and determined the intracellular cation concentrations of salt-adapted and non-salt-adapted cells of both speci...

CONTROL OF MOLECULAR WEIGHT DISTRIBUTION OF THE BIOPOLYMER PULLULAN PRODUCED BY THE FUNGUS Aureobasidium pullulans

Purification and characterization of a new xylanase (APX-II) from the fungus Aureobasidium pullulans Y-2311-1.

Aureobasidium pullulans Y-2311-1 produced four major xylanases (EC 3.2.1.8) with pI values of 4.0, 7.3, 7.9, and 9.4 as revealed by isoelectric focusing and zymogram analysis when grown for 4 days on 1.0% oat spelt xylan. The enzyme with a pI of 9.4 was purified by ammonium sulfate precipitation, chromatography on a DEAE-Sephadex A-50 column, and gel filtration with a Sephadex G-75 column. The ...

Volumetric and Viscometric Studies of Nucleosides, Nucleotides and Furanose Sugar in Aqueous Medium from 288.15 to 298.15K

Density (r/g cm-3) and viscosity (h/10-2gcm-1s-1=1centipoise,CP) of guanosine monophosphate (GMP) and adenosine triphosphate (ATP) referred to as nucleotide, and 2-deoxy adenosine (DOA) and thymidine (TMD) as nucleoside along with their integral furanose sugar, 2-deoxy ribose (DOR) from 0.0004 to 0.0014mol kg<su...

Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces.

A red-shifted, mutated form of the jelly-fish green fluorescent protein (GFP) under control of a TEF promoter was expressed at high levels in the filamentous fungus Aureobasidium pullulans. In the three transformants studied, all morphotypes of the fungus, including pigmented chlamydospores, expressed GFP and fluoresced brightly. Confocal microscopy showed that the intra-cellular distribution o...